The purpose of this study is to find out the effects of relaxing music listening on the negative emotional regulation and the emotional exhaustion on the early childhood teachers. Sixty early childhood teachers working in Seoul, Incheon, Gyeong-gi, and Chung-cheong province participated in this study and were randomly divided into two groups: thirty-one of them were control groups and twenty-nine of them were experimental groups. Experimental groups were participated in 10 times of relaxing music listening for 2 weeks. Every participants were checked their negative emotional regulation by K-PANAS and K-DERS, and emotional exhaustion by MBI before and after the experiment. The results were as follows. First, there were significant differences between two groups in the level of positive and negative emotion. The level of positive emotion significantly increased and the level of negative emotion significantly decreased among experimental groups after relaxing music listening. Second, there were significant differences between two groups in the negative emotional regulation score. The negative emotional regulation score in experimental groups were significantly improved after relaxing music listening. Third, even though the emotional exhaustion score in experimental groups decreased compared to the control groups, there were no significant difference between two groups. The results of this study shows that the relaxing music listening can be a effective emotional supporting method for the early childhood teachers on regulating negative emotion and alleviating emotional exhaustion.