The purpose of this study was to investigate the relationships among job stress, self-care, and psychological burn-out of art therapists, and the mediation effect of the art therapists' self-care on the relationship between job stress and psychological burn-out. The study subject were 128 art therapists who had obtained their bachelor’s degree in art therapy at university or higher, and had been practicing art therapy for more than one year. The research tools used were the job stress scale, psychological burn-out scale, and self-care scale. The data were analyzed using Person’s Correlation Analysis, Regression Analysis, and Hierarchical Multiple Regression Analysis, and The Sobel Test. The study results are summarized as follows: First, there is significant positive correlation between art therapists’ job stress and psychological burn-out, but a significant negative correlation between art therapists’ self-care and job stress and between self-care and psychological burn-out. Second, art therapists’ job stress had a significant influence on psychological burn-out, and job-related factor and interpersonal factor of job stress influenced burn-out significantly. Third, art therapists’ self-care has significant influence on psychological burn-out, and self-regulation behavior, balanced behavior, and identity reinforcing behavior of self-care influenced burn-out significantly. Fourth, the art therapists’ self-care had a partial mediation effect on job stress and psychological burn-out. Implications and future research directions are proposed.